The Effect of Internalized Paramagnetic Nanoparticles on Caenorhabditis elegans Locomotion in the Presence of Magnetic Field

Caenorhabditis elegans nematodes are broadly used to investigate the impact of environmental factors on animal physiology and behavior. Here, C. with internalized paramagnetic nanoparticles were placed inside a magnetic field (MF) explore its effects locomotion. We hypothesized that combined external MF affect elegans’ locomotion machinery. To test our hypothesis, we adult fed bacteria mixed 1 μm, 100, 40 nm diameter. The presence worms’ body (alimentary canal, muscle) was verified by fluorescent electron microscopy. A custom-made software track freely moving in absence or MF, sequentially, for 200 + s. established metrics quantify locomotion-related parameters, including posture, motion, path features. Key attributes (stay ratio, forward over backward speed) affected only worms 100 (reduced speed, increased stay decreased forward/backward ratio), contrast untreated worms. Our work shows particles specific properties under MF. Hence, it contributes clarifying activated locomotion, thus fueling further research.